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Image Search Results
Journal: Medical Molecular Morphology
Article Title: Quantitative volumetric analysis of the Golgi apparatus following X-ray irradiation by super-resolution 3D-SIM microscopy
doi: 10.1007/s00795-020-00277-z
Figure Lengend Snippet: Visualization of RCAS1 signals in X-ray-irradiated cells by super-resolution imaging using 3D-SIM. a Representative images of RCAS1 and DAPI are shown. RPE cells were irradiated with 10 Gy X-rays, fixed 48 h later, and stained with an anti-RCAS1 antibody and DAPI. Images are taken using the 3D-SIM setting in DeltaVision OMX version 4. b Surface polygon images of RCAS1 were generated by Imaris 8.1.2. c Volume of RCAS1 was measured after generation of polygon rendering using imaging software Imaris 8.1.2. RPE cells were irradiated with 10 Gy X-rays, fixed 48 h later, and stained with an anti-RCAS1 antibody and DAPI. Statistical significance was determined using Welch’s t test. *** P < 0.001
Article Snippet: 3D-SIM was performed using a microscope system (
Techniques: Irradiation, Imaging, Staining, Generated, Software